Microarray Gene expression arrays Human endometrial stromal cells

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Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Human - Endometrial stromal cells Target preparation kit (Amplification + Hybridization + control)

Products Thermo Fisher Scientific GeneChip® HT 3' IVT PLUS Reagent Kit

Get tips on using Whole Mouse Genome Microarray Kit, 4x44K to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

Products Agilent Technologies Whole Mouse Genome Microarray Kit, 4x44K

Get tips on using ElectraSense 4x2K/12K to perform Microarray Gene expression arrays - Human whole blood cells Biotin

Products Custom Array ElectraSense 4x2K/12K

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray Comperative genomic hybridization Human Blood cells

Get tips on using Rat GE 4x44K v3 Microarray Kit to perform Microarray Gene expression arrays - Rat pancreas tissue Cyanine 3 & cyanine 5

Products Agilent Technologies Rat GE 4x44K v3 Microarray Kit

Get tips on using Rat GE 4x44K v3 Microarray Kit to perform Microarray Gene expression arrays - Rat cholangio carcinoma cyanine 3 & cyanine 5

Products Agilent Technologies Rat GE 4x44K v3 Microarray Kit

Get tips on using SurePrint G3 Mouse Exon 4x180K Microarray Kit (165,984 Exon probes) to perform Microarray Gene expression arrays - Mouse Cyanine-CTP

Products Agilent Technologies SurePrint G3 Mouse Exon 4x180K Microarray Kit (165,984 Exon probes)

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary human marrow stromal cells

RNA Microarray Human Precision cut lung slices Expression array

Get tips on using Renilla luciferase vector, pGL4.74 to perform Reporter gene assay luciferase - primary human endometrial stromal cells

Products Promega Renilla luciferase vector, pGL4.74

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